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. 2007 May 14;104(21):8924–8929. doi: 10.1073/pnas.0700932104

Fig. 4.

Fig. 4.

Cre/lox-mediated site-specific recombination. (A) Diagram of recombination between the lox71-DsRed of the R2 minichromosome and a P35S-lox66-Cre transgene from J11–9. The CaMV 35S promoter (P35S), Cre gene, DsRed gene, and lox sites (lox66, lox71, lox72, loxP) are indicated. The recombination produces P35S-loxP-DsRed on the donor chromosome that activates the red fluorescence protein expression and lox72-Cre plus other sequences from the donor chromosome to the minichromosome. (B) Red fluorescence protein expression from the recombination of J11–9 and R2 in root tissue (upper) and the absence of red fluorescence protein in the R2 control (lower). (C and D) Sequence alignments of the two recombination products amplified by PCR with sequences of DsRed coding region, lox66, lox71, and P35S-lox66-Cre (C) and the sequences of Cre coding region, lox66, lox71, and pWY86 (D). p1, pr1, p2, and pr2 are outer primers for primary PCR. p1′, pr1′, p2′, and pr2′ are inner primers for nested PCR. The DsRed gene (red arrow), CaMV 35S promoter (P35S, black arrow), Cre gene (black arrow), the linker region between P35S-Bar gene (black arrow), and the lox72 and loxP, which resulted from recombination (red lines), are labeled.