Table 1.
Transmission of minichromosomes
Event | 76-15a | 86-4 | 86B23 | 86B155 | R2 |
---|---|---|---|---|---|
Chromosome size | 1/2 B | 1/5 B | 1/16 B | 3/4 B | NM |
Transgene location | Distal | Distal | Distal | Distal | Distal |
Cross | As male | As male | As male | Self | Self |
Transmission (a) | 14 of 36 | 3 of 25 | 7 of 29 | 9 of 18 | 15 of 46 |
Transmission (b) | 30 of 36 | 6 of 25 | 8 of 29 | 16 of 18 | 16 of 46 |
Chromosome size was visually estimated by comparison of minichromosomes with normal B chromosomes in mitotic cells except for 86B23, whose size was estimated by comparison of pachytene chromosomes with the Image Gauge program (Fuji, Tokyo, Japan). NM, R2 size was not measured. The number of progeny that receive the minichromosome(s) is presented in row (a); the numbers of minichromosomes transmitted to each progeny is presented in row (b). Progeny can receive 2–4 minichromosomes from a male parent by nondisjunction during pollen mitoses in the presence of a normal B chromosome.