Table 2.
Comparison of real-time RT-PCR and array results
| ORF number | Gene name | H2/leu |
H2/Pi |
Rapid/slow (H2-lim) |
Rapid/slow (Pi-lim) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RT-PCR | Array | P value | RT-PCR | Array | P value | RT-PCR | Array | P value | RT-PCR | Array | P value | ||
| Mmp0009 | 0.11 (1.1) | −0.03 (0.98) | 6.5E-01 | 0.08 (1.1) | 0.05 (1.0) | 5.4E-01 | 0.06 (1.04) | −0.02 (0.99) | 6.8E-01 | 0.12 (1.1) | 0.13 (1.1) | 4.9E-02 | |
| Mmp0127 | hmd | 0.00 (1.0) | 0.34 (1.3) | 7.9E-02 | 0.09 (1.1) | −0.20 (0.87) | 2.6E-01 | 1.80 (3.5) | 2.12 (4.3) | 1.1E-08 | 0.82 (1.8) | 0.03 (1.0) | 5.9E-01 |
| Mmp0372 | mtd | 3.13 (8.8) | 4.49 (22.5) | 5.6E-16 | 2.61 (6.1) | 4.14 (17.6) | 2.3E-10 | 0.32 (1.2) | −0.50 (0.71) | 2.2E-02 | 1.79 (3.5) | 1.09 (2.1) | 1.0E-01 |
| Mmp1100 | 2.40 (5.3) | 1.42 (2.7) | 5.4E-06 | 0.10 (1.1) | −0.12 (0.92) | 3.9E-01 | −1.01 (0.50) | −2.39 (0.19) | 4.0E-10 | −0.36 (0.78) | −1.37 (0.39) | 6.2E-07 | |
| Mmp1503 | porE | 0.57 (1.5) | 0.61 (1.5) | 1.1E-05 | 0.07 (1.0) | 0.40 (1.3) | 2.3E-07 | 1.55 (2.9) | 0.64 (1.6) | 2.6E-07 | 1.81 (3.5) | 0.34 (1.3) | 1.9E-01 |
Ratios are log2. Nonlogged ratios are given in parentheses. PCR values are average log2 expression ratios from two biological replicates.