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. 2007 May 11;104(21):8941–8946. doi: 10.1073/pnas.0702860104

Fig. 2.

Fig. 2.

Recovery of carotenoid-rich yellow phenotype by transgenic expression of the CBP gene. (A) Organization of the pBacMCS[UAS-CBP-3xP3-EGFP] vector. ITR, inverted terminal repeats of piggyBac; term, SV40 terminator; 3xP3, eye-specific promoter. (B) The mating system for transgenic expression of the CBP gene using the binary GAL4/UAS system (16). (C) Physical appearance of the transgenic larvae in the wandering stage. We could visually distinguish yellow hemolymph larvae (arrow) from colorless hemolymph larvae (arrowhead) in the rearing container. (Inset) Their hemolymph. (D) A representative chart of the reverse-phase HPLC analysis of the hemolymph carotenoid composition of the transgenic larvae. Detection was at 443 nm. (E) Western blot analysis of CBP expression in the midgut of the transgenic larvae. Lanes 1 and 3, yellow hemolymph; lanes 2 and 4, colorless hemolymph; lane 5, the N4 strain (positive control). (F) A pair of silk glands of the transgenic yellow and control colorless larvae at the wandering stage. Carotenoid uptake occurred in the middle silk gland (MSG) but not in the posterior silk gland (PSG). The boundary between MSG and PSG is indicated by a dotted line. (G) Western blot analysis of CBP expression in the transgenic yellow silk gland shown in F. (H) Cocoons colored by transgenic expression of the CBP gene. Lane 1, the w1-pnd strain as a control white cocoon; lane 2, heterozygotes for the CBP transgene; lane 3, the progeny of sib mating from the strain heterozygous for the CBP transgene. (Scale bar, 1 cm.)