Fig. 1.
Mannose-sensitive entry of FVIII into DC. (A) DC were preincubated with 5 mM EDTA, 1 mg/ml mannan or 1 mg/ml d-galactose for 30 min at 37°C, before the addition of FVIII (40 μg/ml) for 2 h. Reported values depict relative antigen uptake defined as [(37°CMFIinh − 4°CMFImedium)/(37°CMFImedium − 4°CMFImedium)] × 100, where “MFIinh” stands for MFI detected in the presence of the inhibitor. Results are from 12 donors. Statistical significance was calculated on raw data by using unpaired Student's t test. (B) Inhibition of endocytosis in DC by mannan. Preincubation of DC with mannan (1 mg/ml) was followed by addition of dextran-FITC (50 μg/ml), FVIII-FITC (40 μg/ml) or lucifer yellow (LY, 200 μg/ml) for 2 h.