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. 2007 May 14;104(21):8965–8970. doi: 10.1073/pnas.0702120104

Fig. 2.

Fig. 2.

Mannose-sensitive binding of FVIII to CD206. (A) Binding of FVIII to CD206 constructs CTLD4–7-Fc and CR-FNII-CTLD1–3-Fc. ELISA plates were coated with FVIII. CTLD 4–7-Fc and CR-FNII-CTLD1–3-Fc were added to the wells at 10 μg/ml. Fc portions were detected with a mouse anti-human Ig Fc-specific antibody coupled to HRP. (B) Inhibition of the binding of FVIII to CTLD4–7-Fc by mannan. FVIII (5.56 μg/ml) was coated on ELISA plates. Mannan was preincubated with CTLD4–7-Fc (10 μg/ml) for 30 min and loaded on the coated wells.