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. 2007 Apr 6;104(21):8977–8982. doi: 10.1073/pnas.0608703104

Fig. 3.

Fig. 3.

LIRKO mice show massive islet hyperplasia secondary to enhanced β-cell replication. (a) Morphometric analyses of β-cell mass in 10- to 12-month-old control, βIRKO, and LIRKO mice as described in Methods. ∗, P < 0.05 control vs. βIRKO or LIRKO; †, P < 0.01, βIRKO vs. LIRKO (n = 4–6). βIRKO/LIRKO mice were dead by ≈8 weeks. Analyzed by single observer blinded to genotypes. (b) BrdU+ β-cells in 10-month-old control, βIRKO, and LIRKO mice. ∗, P < 0.05 control vs. βIRKO or LIRKO; †, P < 0.01, βIRKO vs. LIRKO (n = 4–6). (c) Representative islets from three male LIRKOs immunostained for insulin (red), BrdU (green), and nuclear stain DAPI (blue). Arrowheads point to BrdU+ cells. (Magnification: ×63.) (Scale bar: 100 μM.) (d) Alterations in blood glucose, serum insulin, and BrdU+ β-cells in 6-week- and 6-, 10-, and 20-month-old control and LIRKO male mice. ∗, P < 0.05 LIRKO vs. control; n = 4–7. Serum insulin is plotted on log scale. BrdU immunostaining was performed as described in Methods.