Fig. 1.

Colocalization of TIA-1 and TIAR with WNV proteins in infected BHK cells. (A–D) Laser scanning confocal microscopy of mock-infected (A) or WNV-infected (MOI of 5) (B–D) BHK cells stained with anti-WNV (red) and anti-TIAR antibody (green) at the indicated times after infection. (E–G) BHK stained with anti-WNV (red) and anti-TIA-1 antibody (green) at the indicated times after WNV (F and G) or mock (E) infection. Nuclear DNA (blue) was stained with Hoechst 33258 dye. (H) Quantification of the amount of TIAR (Left) or TIA-1 (Right) in the nucleus of mock-infected cells (M) and WNV-infected cells at the indicated times after infection. The relative pixel intensity in the nuclei of 20 cells at each time after infection was measured, and the mean values were plotted. Error bars indicate the standard deviation of the mean.