Fig. 2.

Interaction of TIA-1 and TIAR with WNV replication complex components. BHK cells were infected with WNV at a MOI of 0.1. (A–F) Laser scanning confocal microscopy of mock-infected (A and D) and WNV-infected (B, C, E, and F) cells stained with anti-dsRNA (A–C, red) or anti-NS3 (D–F, red) and then with either anti-TIAR (A, B, D, and E, green) or anti-TIA-1 (C and F, green) antibodies at 36 hpi. Enlarged views are included in the B and C merged panels. (G) Coimmunoprecipitation of WNV proteins by anti-TIA-1 and anti-TIAR antibodies. BHK cells were infected with WNV at a MOI of 5. Immunoprecipitates were visualized by Western blot using anti-WNV (Left) or anti-NS3 antibody (Right). Lane 1, mock-infected lysate; lane 2, WNV-infected lysate; lanes 3 and 5, mock-infected immunoprecipitates; lanes 4 and 6, WNV-infected immunoprecipitates. Arrows indicate the positions of the WNV proteins detected. Positions of molecular weight standards are indicated on the left.