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. 2007 Apr 4;35(8):2564–2572. doi: 10.1093/nar/gkm082

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Knock-down of hMtr4p and C1D leads to the accumulation of 5.8S rRNA precursors. HEp-2 cells were transiently transfected with siRNAs directed to PM/Scl-100 (lane 2), MPP6 (lane 3), hMtr4p (lane 4), C1D (lane 5) or with a control siRNA (lane 1). Cells were harvested 2 days after transfection and 2.5 µg of total RNA was analyzed by northern blot hybridization using radiolabeled probes specific for 5.8S rRNA (left) or ITS2 (upper right). As a control, a U6 snRNA probe was used (lower right). The positions of the 5.8S rRNA precursors are indicated by arrows and the position of mature 5.8S rRNA is indicated with an asterisk.