Table 1.

DNaseI mixes of 70 μl aliquots were prepared as detailed

DNaseI condition (units)	0	20	60	120
10× DNaseI buffer (μl)	7	7	7	7
DNaseI (2 U/μl) (Ambion) (μl)	0	10	30	60
Water (μl)	63	53	33	3

The resuspended nuclei were added as 30 μl aliquots to make a final digestion volume of 100 μl.