Table 1.
Summary of errors for synthesis of DsRed in the microfluidic device as compared to in a standard PCR tube
Error type | Microfluidic device | PCR tube | |
---|---|---|---|
Deletion | Single-base | 19 | 16 |
Deletion | Multiple-base | 5 | 5 |
Transition | G/C to A/T | 3 | 6 |
Transition | A/T to G/C | 0 | 2 |
Transversion | G/C to C/G | 0 | 0 |
Transversion | G/C to T/A | 1 | 1 |
Transversion | A/T to C/G | 1 | 0 |
Transversion | A/T to T/A | 0 | 0 |
Total errors: | 29 | 30 | |
Bases sequenced: | 16 250 | 13 389 | |
Error rate (per base): | 0.0018 | 0.0022 |