Table 2.
Calculations for expected oligonucleotide yields from a typical DNA microarray for 16 oligonucleotides, sufficient to build a 400 bp gene. Values for spot area and spot spacing are for commercially available Agilent and Nimblegen DNA microarrays
| Agilent | Nimblegen | |
|---|---|---|
| Area of spot | 1.4 × 104 μm2 | 2.56 × 102 μm2 |
| Oligo density | 0.1 pmol/mm2 | 0.1 pmol/mm2 |
| Maximum expected yield per spot | 1.4 fmol | 0.0256 fmol |
| Dimensions of spot spacing | 212 μm by 188 μm | 25 μm by 25 μm |
| Minimal footprint of 16 oligo spots | 6.4 × 105 μm2 | 1 × 104 μm2 |
| Minimal chamber volume (10 μm height) | 6.4 nl | 100 pl |
| Estimated concentration of each oligo | 220 nM | 256 nM |