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. 2007 Apr 10;35(8):2682–2694. doi: 10.1093/nar/gkm151

Figure 1.

Figure 1.

Anti-TREX2 polyclonal antibody development. (A) Detection of Flag-TREX2 expression in HEK293 cells by anti-TREX2 polyclonal anti-serum. E: pCMV-Flag; T: pCMV-Flag-TREX2. (a) Coommassie stain for total loading of cell lysates; (b) immunoblot by pre-immune serum; (c) immunoblot by anti-TREX2 anti-serum; (d) immunoblot by anti-Flag; (e) anti-Flag immunoprecipitation followed by immunoblot with anti-TREX2 antibody. (B) Test cross-reactivity of anti-TREX2 antibody to TREX1. Empty vector: EV; TREX1: TX1; TREX2: TX2. β-Actin serves as an internal loading control.