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. 2007 Apr 10;35(8):2719–2733. doi: 10.1093/nar/gkm174

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — (A) Effect of the order of addition on S. pombe Dmc1-Hop2-Mnd1 D-loop formation in Mg²⁺ or Ca²⁺ buffer. Reactions with Dmc1 (4 μM) and Hop2-Mnd1 (1 μM) were performed for 5 min (lanes a–d) or 30 min in MgCl₂ (2.5 mM) (lanes e–h) or 5 min (lanes i–l) or 30 min (lanes m–p) in CaCl₂ buffer. (2.5 mM). (B) SpH2M1 does not stimulate spRad51. Indicated concentrations of spRad51 and spH2M1 were used (lanes a–f) and mammalian Dmc1 and Hop2-Mnd1 reaction is shown for comparison (lane g). (C) SpH2M1 interacts with both spDmc1 and spRad51. Purified His-tagged spH2M1 was incubated with affinity beads containing crosslinked BSA (lane a), spRad51 (lane b), or spDmc1 (lane c). Eluted material was probed by western blotting using anti-His antibody.