Figure 8.

Retinal whole mounts of fluorescein dextran-perfused transgene-positive (+) and -negative (−) rho/PDGFB1 mice show that transgene-positive mice have decreased retinal capillary density, vascular malformations, and no neovascularization. Mice were perfused with fluorescein-labeled dextran at P21 and retinal whole mounts were examined by fluorescence microscopy. Transgene-positive mice showed an irregular capillary bed that was less dense than that in transgene-negative mice, and showed moderate sized areas of nonperfused retina (asterisks). No neovascularization was identified, but there were vascular malformations (arrows). B and C are higher power views of the same retina shown in A, and E and F are higher power views of the same retina shown in D.