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. 2000 Sep;157(3):945–955. doi: 10.1016/S0002-9440(10)64607-X

Figure 8.

Figure 8.

Egg/IL-12-sensitized IFN-γ-deficient mice fail to develop an exacerbated inflammatory response, despite exhibiting a marked NOS-2 deficiency. WT C57BL/6 and C57BL/6Ai-[KO] IFN-γ mice were sensitized and infected as described previously. A: Expression of NOS-2 mRNA in inflammatory liver tissue was analyzed by semiquantitative RT-PCR. Each data point represents the result of an individual mouse and the bar denotes the group median (n = 9–10). B: Granuloma size was measured in the livers of infected mice. C and D: Production of IL-5 (C) and IFN-γ (D) by in vitro restimulated splenocytes was measured in cell supernatants by ELISA. Each bar in C and D represents the results of pooled samples from all mice in an experimental group. E: Fibrosis was quantified in granulomatous liver tissue by analysis of hydroxyproline content. Data points in E show the mean of duplicate measurements and the bar indicates the group median. Statistical significance between experimental groups was determined by analysis of covariance (P < 0.05). Similar results were produced in a second experiment.