Figure 2.
Western immunoblotting with PAX2 antibody for various cell lines. Lanes 1–3: Endogenous expression of PAX2 protein in mIMCD-3, COS-7 and HEK293 kidney cell lines, respectively. Lanes 4 and 5: Expression of exogenous PAX2 protein in HEK293 tTA/PAX2 cells grown in the absence or the presence of 1 μg/ml of tetracycline. Lanes 6 and 7: Expression of exogenous PAX2 protein under regulation of the tetracycline-sensitive promoter in MDCKtTA/hPAX2 cells in the absence or presence of tetracycline.