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. 2000 Sep;157(3):843–857. doi: 10.1016/S0002-9440(10)64598-1

Figure 5.

Figure 5.

Western blot of Bax (A) and Bcl-2 (B) in NS- and S-noninfected (NI), and AdLacZ- and Adp53m-infected myocytes (n = 6 in all cases). A: OD in noninfected myocytes: NS = 0.88 ± 0.12; S = 2.04 ± 0.21; AdLacZ-infected myocytes: NS = 0.82 ± 0.20; S = 2.22 ± 0.72; Adp53m-infected myocytes: NS = 0.93 ± 0.13; S = 0.03 ± 0.02. B: OD in noninfected myocytes: NS = 0.66 ± 0.16; S = 0.04 ± 0.02; AdLacZ-infected myocytes: NS = 0.71 ± 0.13; S = 0.03 ± 0.02; Adp53m-infected myocytes: NS = 0.64 ± 0.10; S = 1.25 ± 0.25. C: Immunoprecipitation (IP) with Bax antibody (Ab) and Western blot (WB) with Bcl-2 antibody of myocyte lysates obtained from NS- and S-noninfected (NI), and AdLacZ- and Adp53m-infected myocytes (n = 6 in all cases). OD of Bcl-2 bound to Bax in noninfected myocytes: NS = 5.2 ± 1.0; S = 1.1 ± 0.3; AdLacZ-infected myocytes: NS = 4.9 ± 1.2; S = 0.9 ± 0.2; Adp53m-infected myocytes: NS = 5.3 ± 1.5; S = 0.03 ± 0.02. D: IP with Bcl-2 Ab and WB with Bax and Bcl-2 Ab of myocyte lysates obtained from NS- and S-noninfected (NI), and AdLacZ- and Adp53m-infected myocytes (n = 6 in all cases). OD of the total Bcl-2 in noninfected myocytes: NS = 3.6 ± 0.7; S = 0.02 ± 0.02; AdLacZ-infected myocytes: NS = 3.3 ± 0.6; S = 0.02 ± 0.01; Adp53m-infected myocytes: NS = 3.9 ± 1.1; S = 11.5 ± 3.0. OD of Bax bound to Bcl-2 in noninfected myocytes: NS = 1.6 ± 0.4; S = 0.03 ± 0.01; AdLacZ-infected myocytes: NS = 1.8 ± 0.5; S = 0.02 ± 0.01; Adp53m-infected myocytes: NS = 1.5 ± 0.5; S = 0.02 ± 0.02.