Table 2.

Efficiency of Housekeeping and Target Gene mRNA Detection in Lungs Undergoing Immunofluorescence Labeling

	PBGD-positive picked samples	TNF-α-positive picked samples	NOSII-positive picked samples	PBGD threshold cycle CT ± SEM
Control lung	42 /61	5 /28	0 /33	37.02 ± 0.40
Stimulated lung 1	37 /68	17 /47	8 /21	37.18 ± 0.22
Stimulated lung 2	42 /85	17 /35	16 /50	37.24 ± 0.21

Per sample 15 AM profiles were picked, and the number of samples positive for the investigated mRNA is given in relation to the total number of samples (= efficiency rate). The threshold cycles (CT) for PBGD mRNA varied only slightly, thus confirming the presence of similar amounts of mRNA within the samples.