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. 2000 Nov;157(5):1703–1711. doi: 10.1016/S0002-9440(10)64807-9

Figure 2.

Figure 2.

Effect of ET-1 and VEGF on HUVEC migration. Endothelial cells (5 × 10 5 cells/ml) pretreated for 15 minutes with 1 μmol/L of the ETAR (BQ 123) or ETBR antagonist (BQ 788) were placed in the upper compartment of a 48-well Boyden chamber. VEGF (1 ng/ml), ET-1 (10 nmol/L), and ETBR agonists (ET-3 and S6c, 10 nmol/L) were added in lower wells. Cells migrated through the filter were counted after 4 hours. Data are expressed as the number of migrated cells in 10 high-power fields and are the means of results from three experiments each performed in triplicate. Bars, SD. a: P ≤ 0.02 compared to control. b: P ≤ 0.001 compared to control. c: P ≤ 0.02 compared to ET-1. d: P ≤ 0.01 compared to VEGF.