Figure 3.
Effect of ET-1 and VEGF on HUVEC invasion. HUVEC (5 × 10 5 cells/ml) were seeded on Matrigel layer in a Boyden chamber assay. Endothelial cells were preincubated for 15 minutes with ETA (BQ 123) or ETB (BQ 788) receptor antagonists (1 μmol/L) before the addition of 100 nmol/L ET-1. VEGF (1 ng/ml) in the absence or presence of ET-1 (100 nmol/L) was added in the lower wells. Cells migrating through the filter were counted after 6 hours. Data are expressed as the number of migrated cells in 10 high-power fields and are means of results from three experiments each performed in triplicate. Bars, SD. a: P ≤ 0.001compared to control. b: P ≤ 0.001 compared to ET-1. c: P ≤ 0.01 compared to VEGF.