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. 2000 Nov;157(5):1623–1631. doi: 10.1016/S0002-9440(10)64800-6

Figure 1.

Figure 1.

Gene expression in HPDE6-E6E7 cells and their clones. A: Reverse transcriptase-polymerase chain reaction analyses showed the mRNA expression of carbonic anhydrase-II and MUC-1 genes in the pre-immortalized HPDE6-E6E7 cells and its immortal clones (c2–c11). B: cDNA microarray hybridization was used to demonstrate the expression of various cytokeratin mRNA in HPDE6-E6E7c7 cells. C: The immortal HPDE6-E6E7 (P17) cells and the clones expressed high levels of p16 mRNA expression, whereas the primary cultured HPDE6 cells that were not exposed to the E6E7 retrovirus showed low levels of p16 mRNA. D: Southern blot analysis confirmed the presence of same copy number of p16, p53, and c-myc genes in the various HPDE6-E6E7 clones.