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. 2000 Nov;157(5):1623–1631. doi: 10.1016/S0002-9440(10)64800-6

Figure 6.

Figure 6.

The Rb pathway in HPDE cells. A: The HPDE6-E6E7c7 cells demonstrated no detectable pRb/p110 level, but p130 was present at high levels. These cells also demonstrated Smad-2/3 and Smad-4 protein expression. As comparison and positive control for Rb/p110, the human bronchial epithelial cells were similarly analyzed. B: The HPV16-E7 protein was expressed in HPDE6-E6E7c7 cells and Kaski cervical squamous cell carcinoma cell line, but not in NIH3T3 cells. C: Treatment of the HPDE6c7 cells by 20 ng/ml of TGF-β1 induced up-regulation of p21 protein as early as 3 hours after treatment, but induction of p27 was delayed up to later than 6 hours. As expected, TGF-β1 failed to modulate the levels of cyclin D3, cyclin B, and cdk4.