Table 1.

Primers Used for the Amplification and Construction of a cRNA Standard from EIF3S8 and TBP and Fluorescence Hybridization Probes for Quantification of EIF3S8 and TBP mRNA Expression in Tissue

Primer	EIF3	TBP
5′-PCR primer	5′-GCTAAGAAGAAGCACGACAGGAAAT-3′	5′-GCTCTTCCACTCACAGACTC-3′
3′-PCR primer	5′-CCAGGTTGGGGTTGTAGTCATAG-3′	5′-GCCAGTCTGGACTGTTCTTC-3′
5′-cRNA construction-primer	5′-GGATCCTAATACGACTCACTATAGGGAGG GCTAAGAAGAAGCACGACAGGAAAT-3′	5′-GGATCCTAATACGACTCACTATAGGGAGG GCTCTTCCACTCACAGACTC-3′
3′-cRNA construction-primer	5′-TTTTTTTTTTTTTTTCCAGGTTGGGGTTG TAGTCATAG-3′	5′-TTTTTTTTTTTTTTTGCCAGTCTGGACTG TTCTTC-3′
FL-hybridization probe	5′-GACCCTTTCCCACTCCCCGCCTT-FL-3′	5′-CCGTGGTTCGTGGCTCTCTTATCCTC-FL-3′
LCR-hybridization probe	5′-LCR-TGTCCTCCTCCTCCTCATCCAGGC-3′	5′-LCR-TGATTACCGCAGCAAACCGCTTG-3′

FL, fluorescein; LCR, light-cycler-red 640.