Figure 3.

Left: FISH micrograph showing an example of case 178. Hybridization was simultaneously performed with a probe for chromosome 8 (present in either two or four copies) and chromosome 11 (present in either one or two copies). Indeed we could confirm the presence of two populations. An example of the smallest population, containing four copies of chromosome 8 and two copies of chromosome 11, is shown. Right: FISH on paraffin slides using a sex chromosome (X) centromere probe on a male patient (case 408) is demonstrated. One copy of the X centromere is demonstrated in both nuclei of binucleated cells, indicating that a normal mitosis precedes the formation of these cells, which are highly characteristic for chondrosarcoma. A meiosis-like division in binucleated cells is therefore most probably not the cause of near-haploidy in chondrosarcoma.