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Analysis of phospho MAP kinase and total MAP kinase expression in immortalized (MS1) and transformed endothelium (SVRbag4) in the presence and absence of a chemical MAPKK inhibitor (PD98059), and cell lines (SVRA221a,b,c) expressing a dominant negative MAPKK gene. The top gel represents an immunoblot with an antibody specific for phosphorylated MAP kinase, and the bottom gel represents an immunoblot with an antibody for total MAP kinase.
