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Effect of MAPKK inhibition on metalloproteinase bioactivity in SVR-conditioned media. Conditioned media from equal numbers of cells were electrophoresed through an acrylamide gel containing gelatin. White bands represent gelatinolysis. The first two lanes represent SVR cells treated with vehicle (DMSO) or PD98059; the second two lanes compare a vector control (SVRbag4) with a clone expressing a dominant negative (SVRA221a). Experiments were repeated in triplicate.
