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A representative soft agar assay stained with 0.2% Giemsa 2 weeks after plating. MS1, SVRbag4 treated with 50 μmol/L PD98059 or vehicle control, and SVRA221a,b cells were seeded at either 5 × 10 4 cells per 6-cm 2 dish. Macroscopic colonies were counted 14 days after plating the cells in agar.
