Immunohistochemical localization of inducible Hsp 70 expression using fluorescein isothiocyanate-labeled secondary antibodies in villi at 6 weeks (a), 9 weeks (b), and 13 weeks gestational age (c). d: Negative control and nitrotyrosines with chromogenic substrate at 6 weeks (e), 9 weeks (f), and 13 weeks gestational age (g). h: Negative control. There is strong immunolabeling for both at 9 weeks, particularly in the syncytiotrophoblast (arrows).