Table 5.

Microscopic Composition of Hepatocyte Ploidy Subpopulations

Experiment no.*	Subpopulation	Percentage nonparenchymal cells X±SD	Percentage doublets† X±SD
3, 4	Diploid‡	4.7	7.3
	Tetraploid	2.6 ± 0.2	8.1 ± 0.4
	Octaploid	2.7 ± 2.5	6.5 ± 1.8
5, 6	Diploid	ND§	ND
	Tetraploid	2.9 ± 0.2	2.2 ± 1.6
	Octaploid	0.4 ± 0.4	3.2 ± 0.9

*Cells isolated in experiments 3 and 4 were transplanted into MUP-uPA recipient mice and assessed for repopulation efficiency (Table 6) ▶ .

^†Cells isolated in experiments 3 and 4 were held in Eppendorf tubes until the experiment’s end, so that postsort clumping could have occurred. Cells isolated in experiments 5 and 6 were sorted directly onto microscope slides. Thus, measured values from experiments 5 and 6 should provide a more accurate measure of the true percent of cell clumping that is present immediately after sorting using this cell isolation approach.

^‡Data for the diploid subpopulation available only from experiment 3.

^§ND, not determined because the diploid cell population was too low in these cell preparations to permit accurate isolation.