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. 2000 Dec;157(6):1927–1935. doi: 10.1016/S0002-9440(10)64831-6

Figure 2.

Figure 2.

Up-regulation of matrix MMPs in SCID mice with colitis. Zymography was performed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels containing gelatin as substrate and incubated overnight at 37oC in the presence of PMSF, leupeptin, and soybean trypsin inhibitor. A: Colonic extracts from eight SCID mice transplanted with CD4+ T cells. B: Colonic extracts from four C.B-17 mice 9–12 and two nontransplanted SCID mice. 13,14 Arrowheads depict MMP-2 standard. Three samples per mouse: distal colon, left; mid colon, middle; proximal colon, right. Relative mobility of the major protease species is given. C: Relative activity of total MMPs and of pro- and active forms of MMP-2 and MMP-9 in the three groups of mice, mean densitometry values of all samples in each group + SE. D: Proportion of MMP-2 in its activated form in colon tissues from mice transplanted with CD4+ T cells (n = 8), nontransplanted SCID mice (n = 2) and C.B-17 mice (n = 4), + SE, as determined by densitometry from gelatin sodium dodecyl sulfate-polyacrylamide gel electrophoresis zymography. E: Relative activity of total MMPs and of pro- and active forms of MMP-2 and MMP-9 in distal, mid, and proximal regions of the colon in CD4+ T-cell-transplanted SCID mice only, mean of densitometry values from all eight mice + SE.