Table 2.
Immunoglobulin Isotypes and Subclasses Expressed by Adenoid and Tonsillar B Cells
| Positive cells (percent) | |||
|---|---|---|---|
| T cell subsets | Tonsils | Tonsillar IELs | Adenoids |
| CD3+ | 29.2 ± 3.5 (12)* | 38.4 ± 4.0 (4) | 34.4 ± 2.5 (6) |
| CD4+ | 20.8 ± 2.5 (9)* | 31.2 ± 1.6 (4) | 28.2 ± 1.1 (4) |
| CD8+ | 5.2 ± 1.4 (9) | 7.5 ± 2.0 (4) | 6.3 ± 0.7 (4) |
| CD3+/ CD4+ | 20.2 ± 1.2 (5) | 27.3 ± 1.2 (3) | 24.3 ± 2.6 (3) |
| CD3+/ CD8+ | 3.9 ± 1.2 (5) | 4.7 ± 2.0 (3) | 6.2 ± 0.7 (3) |
| TCR αβ+ | 25.0 ± 3.3 (9)* | 30.1 ± 1.5 (4) | 35.0 ± 1.5 (4) |
| TCR + | 2.2 ± 1.6 (9) | 2.7 ± 1.8 (4) | 1.8 ± 1.1 (4) |
| CD25+ | 3.3 ± 2.2 (9)* | 5.1 ± 1.7 (4) | 7.8 ± 1.6 (4) |
| CD28+ | 23.5 ± 1.6 (9)* | 38.3 ± 2.5 (4) | 36.1 ± 2.6 (4) |
The T cells subsets in adenoids and tonsils were determined by incubating cell suspensions with the appropriate FITC-labeled and/or PE-labeled mAbs as described in Materials and Methods. After washing and fixation with 1% paraformaldehyde, the cells were analyzed by flow cytometry. The results are expressed as the mean percentage ± one SD. The number of samples is indicated in parentheses. *P < 0.05 when compared with adenoids.