Figure 2.

Activated gelatinase A is present in the culture supernatants of gut mesenchymal cells after α4β1 ligation. Mesenchymal cells were stimulated with different concentrations of anti-VLA4 or VCAM-IgG for 2 days. Culture supernatants were collected and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and Western blotting. Human IgG was used as negative control whereas IL-1β and TNF-α were used as positive controls. Arrows pointing to specific bands denote latent and active forms of MMPs. This is a representative figure from five different experiments.