Figure 3.

Ezrin-IR in glial tumors. Immunostaining was performed using the indirect immunoperoxidase method with diaminobenzidine as the chromogen and hematoxylin for counterstaining. Staining intensity was evaluated at 20 × light-microscopic magnification. The scoring scale was defined as follows: 0, no staining; 0.5, single weakly positive cells between large numbers of entirely negative cells (<5% of the tumor cells); 1, delicate staining of astrocytic processes in normal tissues or for weak but staining in at least 50% of tumor cells with distribution similar to the normal in individual tumor cells; 2, increased staining of 50 to 70% of the tumor cells; 3, strongly increased staining of at least 80% of the tumor cells. Intermediate levels were scored 1.5 and 2.5, respectively. The scoring values were chosen to yield an average score value of 1 in normal tissues.