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. 2007 May 16;7:47. doi: 10.1186/1471-213X-7-47

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Copyright © 2007 Saka and Smith; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mutually exclusive expressions of Xbra and Gsc in Xenopus animal pole blastomeres. (C) Mutually exclusive expressions of Xbra and Gsc in Xenopus animal pole blastomeres. Fertilised Xenopus embryos were injected with RNA encoding HA-tagged Gsc (Gsc-HA). Animal pole regions derived from these injected embryos and from uninjected embryos were mixed, dissociated and treated with 0.5 U/ml of activin. They were fixed after 7 hours of culture and stained with Hoechst 33342 (DNA) and processed for anti-Xbra and anti-HA staining. Scale bar = 20 μm. (D) Arbitrary fluorescence levels of anti-Xbra and anti-HA (Gsc) staining shown in (C) were calculated as described in Methods.