Table 2.

Position (base in bold) and type of paired base mismatch(es) between primer pair LMWF3/LMWR4 and eight BAC clone templates.

BAC clone (template)	LMWF3 (5'→3') GACAAGTGCCATTGCACAGA	Primer-template mismatch	LMWR4 (5'→3') GCTGTACAACGGCACATTGA	Primer-template mismatch	N-terminal sequence
TaE0503E07	GACAAGTGCCATTGCACAGA	No mismatch	GCTGTACAACGGCACATTGA	No mismatch	METSCISG
TaE1238L16	GACAAGTGCCATTGCACAAA	G:Ta	GCTGTACAACGGCACGTTGA	A:C	METSRVPG
TaE0154F22	GACAAGTGCCATTGCACAAA	G:T	TCTATACAACGGCACATTGA	G:A, G:T	MENSHIPG
TaE0359D24	AACAAGTGCCATTGCACAAA	G:T (2×)	TCTGTACAACGGCACATTGA	G:A	IENSHIPG
TaE1157C20	GACAAGTGCCATTGCACAAA	G:T	GCCGTACACCGGCACGTTGA	T:G, A:G, A:C	METSHIPS
TaE0703A09	GACAAGTGCCATTGCACAAA	G:T	GCCGTACACCGGCACGTTGA	T:G, A:G, A:C	METSHIPG
TaE0099E23	GACATCTGCCATTGCACAGA	A:A, G:G	GCTGTACAACGGCACATTGA	No mismatch	METSCIPG
TaE0072P19	GACATCTGCCATTGCACAGA	A:A, G:G	GCTGTACAACGGCACATTAA	G:T	METSCIPG

^aThe first base from the primer and the second base from complementary sequence of template.