Figure 2.

Ca²⁺-Channel Blockers or Deletion of CaCch1p or Either Calcineurin Subunit Attenuates Cathodal Germ-Tube Emergence of C. albicans Hyphae

(A) Cells were grown in an electric field and the medium supplemented with 250 μM verapamil, 100 μM nifedipine, or 500 μM diltiazem. Emergence angles (black bars) and final angles (hatched bars) were measured in relation to the cathode for >100 hyphae per strain per experiment so that percentage cathodal orientation could be obtained [20].

(B) Mutant and control strains were exposed to an electric field of 10 V/cm for 6 hr in Ca²⁺-depleted medium containing glucose (black bars), maltose (conditional mutants, gray bars), or 5 μg/ml FK506, an inhibitor of calcineurin. Conditional MRP1p-regulated mutants were subcultured for 3 days in glucose containing medium prior to assaying.

Each error bar shows the SD of the mean values obtained from three independent experiments.