Figure 3.

The Thigmotropic Response Is Attenuated in Calcium-Signaling-Pathway Mutants

(A) Mutant strains were adhered to quartz slides with ridge height 0.79 μm and pitch 25 μm and grown in 20% (v/v) fetal-calf serum supplemented with 2 (w/v) glucose (black bars), maltose (conditional mutants, gray bars), or a maximal concentration of 10 μg/ml FK506. The number of hypha-ridge interactions resulting in hyphal reorientation was expressed as a percentage of the total number of interactions observed. Each error bar shows the SD of the mean values obtained from three independent experiments.

(B and C) In wild-type cells, 60% of interactions between growing tips and the 0.79 μm ridges in the substrate resulted in reorientation of the hyphal growth axis. In mutant strains, approximately 70% of interactions resulted in hyphae maintaining their direction of growth over the ridges. Scale bars represent 10 μm.