Markers for the Plus Ends of Microtubules Colocalize with Ectopic oskar mRNA
(A–C) A wild-type egg chamber; Kin:β-GAL (A) localizes to the posterior pole of the oocyte at stage 9 and colocalizes with Staufen (B).
(D–F) A UAS-osk egg chamber; Kin:β-GAL (D) and Staufen (E) are mislocalized to a dot in the center of the oocytes with overexpressed oskar mRNA, as well as to the posterior pole.
(G–L) Dynamitin:GFP (G) fusion protein localizes to the posterior pole in wild-type egg chambers and colocalize with pole-plasm markers, such as Stau (H). In UAS-osk flies, Dynamitin:GFP (J) and Stau (K) are mislocalized to the ectopic site in the middle of the oocyte.