Figure 4.

PAR-1 May Mediate Plus-End Recruitment by Oskar Protein

(A–C) Wild-type: An immunostaining for PAR-1 ([A], red in [C]) shows its localization to the posterior with GFP-Staufen ([B], green in [C]).

(D–F) UAS-osk: Overexpression of oskar mRNA leads to the recruitment of PAR-1 ([D], red in [F]) and GFP-Staufen ([E], green in [F]) to the middle of the oocyte.

(G) An immunostaining for Stau protein shows its localization to the posterior pole of a wild-type oocyte.

(H) In the hypomorphic mutant combination, par-1⁶³²³/ par-1⁶⁸²¹, some Staufen is occasionally mislocalized to the middle of the oocyte.

(I) In UAS-osk/GAL4, the overexpression of oskar mRNA also induces mislocalization of Staufen to the oocyte center.

(J) par-1⁶³²³/ par-1⁶⁸²¹; UAS-osk: oskar mRNA overexpression strongly enhances the weak par-1 phenotype, leading to the complete loss of Staufen localization to the posterior.