Figure 2.

DSas-6 Is Required for Centriole Replication

(A and B) The distribution of basal bodies (revealed here with GFP-PACT) in WT (A) and mutant (B) third antennal segments. Basal bodies are present at the base of each sensory bristle in WT antenna (arrow) but are undetectable in mutant antenna.

(C and D) The localization of the centriolar marker DSas-4 (red) and the PCM marker Cnn (green) in WT (C) and DSas-6 mutant (D) mitotic larval brain cells. No centrioles or centrosomes are detectable in the mutant cells.

(E) Quantitation of centriole numbers (D-PLP-positive dots) in WT and DSas-6 mutant mitotic cells (see also Figure S3). More than 70% of mutant cells have no detectable centrioles.

Scale bars represent 10 μm.