Fig. 2.

TAP mediated pull-down of the Arp2/3 complex. Extracts of Dictyostelium cells transformed with vector pDV-CTAP, pDV-CGFP-CTAP or p34-Arc-CTAP were subjected to the TAP purification protocol and size-fractionated by electrophoresis on SDS–PAGE. Gels were stained with colloidal blue and the eight protein bands that were purified from the p34-Arc-CTAP transformed cells were cut out and subjected to tryptic peptide fingerprinting by mass spectrometry. The number of different peptides obtained for each band is indicated in parentheses. Query of the Uniprot protein database with the obtained peptide sequences yielded all 7 members of the D.discoideum Arp2/3 complex and discoidin B.