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Expression of wild types, chimeric, and mutated NaPi cotransporters in OK cells. Cells were transfected with wt mIIa and mIIb, with the chimeras mIIaN-mIIb, mIIbN-mIIa, mIIa-mIIbCT, and mIIb-mIIaCT or with the mutants mIIaNI and mIIbKR. They were then processed for confocal microscopy. (a) Focal planes. (b and c) The xy cross-sections. The endogenous fluorescence of EGFP is shown in green (b) and the actin staining in red (c); a merge of the fluorescence signals is shown in a.
