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SDS/PAGE of samples from each stage in the purification of SPP from rice leaves. Amounts of protein loaded were as follows: lane A, crude extract (15 μg); lane B, 29% polyethylene glycol-8000 precipitate (10 μg); lane C, Sephacryl S300 pooled fractions 12–15 (10 μg); lane D, Brilliant Red-Sepharose 6B fraction 12 (5 μg); lane E, Mono Q fraction 14 (10 μg); lane F, Superdex 200 fraction 30 (0.5 μg).
