Alignment of the deduced amino acid sequence of the maize SPP with
SPP-like sequences and the carboxyl terminus of SPS from other species.
The GenBank accession numbers of the sequences are as follows: maize
(Zea mays) SPP (ZmSPP), AF283564; A.
thaliana SPP (AtSPP), AF283563; Synechocystis
sp. PCC6803 SPP (SynSPP), AF300455; Synechocystis sp.
PCC6803 SPS (SynSPS), D64006 (residues 459–720); and maize SPS
(ZmSPS), M97550 (residues 770-1068). Sequences were aligned using the
PILEUP program of the GCG Wisconsin sequence analysis
package. Residues conserved in five, three, and two out of the five
sequences are highlighted in black, dark gray, and light gray,
respectively. Asterisks indicate residues that are homologous to
conserved residues in motifs I, II, and III of the HAD-type
phosphatases. The sequences of peptides 1, 2, and 3 from purified rice
leaf SPP are also shown in alignment with the SPP sequences.