Table 1.

Purification of SPP from rice leaves

Fraction	Activity, μmol min−1	Specific activity, μmol min−1 mg−1	Purification, -fold
Leaf crude extract	408	0.05	—
50–62% sat (NH4)2SO4	270	0.08	1.6
29% PEG, pH 5	188	1.03	21
S300 (#12–15)	119	1.41	28
Brilliant Red G-E	12*	29	580
Mono-Q	8.5*	520	10,000
Superdex 200	0.96*	1250†	25,000

^*Only part of the available activity from the preceding step was processed. Total recoveries of activity from the Brilliant Red G-E, MonoQ, and Superdex 200 columns were 90, 94, and 95%, respectively. 

^† Protein estimated from the intensity of Coomassie staining of bands on SDS/PAGE gels.