Table 2.
Additions | Activity, μmol min−1 ml−1 |
---|---|
8 mM MgCl2* | 2.95 |
None | 0.34 |
13 mM EDTA | 0.01 |
1 mM MgCl2 | 2.96 |
1 mM MgCl2 + 13 mM EDTA | 0.05 |
0.1 mM MgCl2 | 2.80 |
0.01 mM MgCl2 | 1.61 |
0.002 mM MgCl2 | 0.75 |
Purified SPP was desalted by passage through a Sephadex G-25 column equilibrated with 25 mM Hepes-K+, pH 7.0, and assayed for activity with 1.2 mM Suc6P and various concentrations of MgCl2 or EDTA as shown.
Enzyme before desalting on Sephadex G-25.