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Expression of maize SPP in E. coli
| Substrate | Phosphatase activity, nmol
min−1 mg−1 protein
|
|
|---|---|---|
| Control | SPP | |
| Suc6P | 3 | 370 |
| Suc6P + EDTA | 2 | 53 |
| Fru6P | 14 | 10 |
The putative maize SPP cDNA clone was expressed in E. coli strain ER2566. Phosphatase activity with either 1.25 mM Suc6P or Fru6P as the substrate was assayed in extracts from cells carrying the empty expression vector pTYB4 (control) or pTYB4/ZmSPP1. SPP activity was also measured in the presence and absence of 20 mM EDTA.
