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. 1991 Dec;139(6):1425–1434.

Iron translocation by free fatty acids.

M W Qian 1, J W Eaton 1
PMCID: PMC1886463  PMID: 1750512

Abstract

Organic extracts of cigarette smoke and uncombusted tobacco contain substances capable of translocating iron from aqueous solutions into immiscible organic solvents. Such extracts will also effect the organic solvation of iron present in ferruginous forms of asbestos such as amosite and crocidolite (Qian and Eaton, Arch Biochem Biophys 1989, 275:280). These substances, previously detected by their iron-translocating properties, have now been purified and identified by mass spectroscopy as saturated fatty acids, predominantly stearic and palmitic acids. Organic extracts of tobacco smoke, as well as the pure fatty acids, also transfer ferrous iron into both isolated red cell membranes and intact human erythrocytes. The increased membrane iron may enhance cellular susceptibility to exogenous oxidants; erythrocyte membranes subject to fatty acid-mediated iron accumulation show elevated peroxidation of endogenous polyunsaturated fatty acids. These observations may help explain the phlogistic effects of tobacco use and suggest, in a broader context, that free fatty acids may act as physiologic and pathologic mediators of metal translocation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artiss J. D., Vinogradov S., Zak B. Spectrophotometric study of several sensitive reagents for serum iron. Clin Biochem. 1981 Dec;14(6):311–315. doi: 10.1016/s0009-9120(81)91065-1. [DOI] [PubMed] [Google Scholar]
  2. Buege J. A., Aust S. D. Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302–310. doi: 10.1016/s0076-6879(78)52032-6. [DOI] [PubMed] [Google Scholar]
  3. Church D. F., Pryor W. A. Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect. 1985 Dec;64:111–126. doi: 10.1289/ehp.8564111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Falholt K., Lund B., Falholt W. An easy colorimetric micromethod for routine determination of free fatty acids in plasma. Clin Chim Acta. 1973 Jun 28;46(2):105–111. doi: 10.1016/0009-8981(73)90016-8. [DOI] [PubMed] [Google Scholar]
  5. Gilks B., Wright J., Churg A. Effects of cigarette smoke on tissue uptake and retention of iron oxide in the guinea pig. Am Rev Respir Dis. 1988 Jun;137(6):1382–1384. doi: 10.1164/ajrccm/137.6.1382. [DOI] [PubMed] [Google Scholar]
  6. Hecht S. S., Carmella S., Mori H., Hoffmann D. A study of tobacco carcinogenesis. XX. Role of catechol as a major cocarcinogen in the weakly acidic fraction of smoke condensate. J Natl Cancer Inst. 1981 Jan;66(1):163–169. [PubMed] [Google Scholar]
  7. Jackson J. H., Schraufstatter I. U., Hyslop P. A., Vosbeck K., Sauerheber R., Weitzman S. A., Cochrane C. G. Role of oxidants in DNA damage. Hydroxyl radical mediates the synergistic DNA damaging effects of asbestos and cigarette smoke. J Clin Invest. 1987 Oct;80(4):1090–1095. doi: 10.1172/JCI113165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ko K. M., Godin D. V. Ferric ion-induced lipid peroxidation in erythrocyte membranes: effects of phytic acid and butylated hydroxytoluene. Mol Cell Biochem. 1990 Jun 25;95(2):125–131. doi: 10.1007/BF00219970. [DOI] [PubMed] [Google Scholar]
  9. Krause G. S., Nayini N. R., White B. C., Hoenher T. J., Garritano A. M., O'Neil B. J., Aust S. D. Natural course of iron delocalization and lipid peroxidation during the first eight hours following a 15-minute cardiac arrest in dogs. Ann Emerg Med. 1987 Nov;16(11):1200–1205. doi: 10.1016/s0196-0644(87)80224-x. [DOI] [PubMed] [Google Scholar]
  10. Low R. B., Davis G. S., Giancola M. S. Biochemical analyses of bronchoalveolar lavage fluids of healthy human volunteer smokers and nonsmokers. Am Rev Respir Dis. 1978 Nov;118(5):863–875. doi: 10.1164/arrd.1978.118.5.863. [DOI] [PubMed] [Google Scholar]
  11. McGowan S. E., Henley S. A. Iron and ferritin contents and distribution in human alveolar macrophages. J Lab Clin Med. 1988 Jun;111(6):611–617. [PubMed] [Google Scholar]
  12. McGowan S. E., Murray J. J., Parrish M. G. Iron binding, internalization, and fate in human alveolar macrophages. J Lab Clin Med. 1986 Dec;108(6):587–595. [PubMed] [Google Scholar]
  13. Mordelet-Dambrine M. S., Stanislas-Leguern G. M., Huchon G. J., Baumann F. C., Marsac J. H., Chrétien J. Elevation of the bronchoalveolar concentration of angiotensin I converting enzyme in sarcoidosis. Am Rev Respir Dis. 1982 Sep;126(3):472–475. doi: 10.1164/arrd.1982.126.3.472. [DOI] [PubMed] [Google Scholar]
  14. Nakayama T., Church D. F., Pryor W. A. Quantitative analysis of the hydrogen peroxide formed in aqueous cigarette tar extracts. Free Radic Biol Med. 1989;7(1):9–15. doi: 10.1016/0891-5849(89)90094-4. [DOI] [PubMed] [Google Scholar]
  15. Pacht E. R., Kaseki H., Mohammed J. R., Cornwell D. G., Davis W. B. Deficiency of vitamin E in the alveolar fluid of cigarette smokers. Influence on alveolar macrophage cytotoxicity. J Clin Invest. 1986 Mar;77(3):789–796. doi: 10.1172/JCI112376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pryor W. A. Cigarette smoke and the involvement of free radical reactions in chemical carcinogenesis. Br J Cancer Suppl. 1987 Jun;8:19–23. [PMC free article] [PubMed] [Google Scholar]
  17. Qian M. W., Eaton J. W. Tobacco-borne siderophoric activity. Arch Biochem Biophys. 1989 Nov 15;275(1):280–288. doi: 10.1016/0003-9861(89)90374-3. [DOI] [PubMed] [Google Scholar]
  18. Simpson R. J., Moore R., Peters T. J. Significance of non-esterified fatty acids in iron uptake by intestinal brush-border membrane vesicles. Biochim Biophys Acta. 1988 Jun 7;941(1):39–47. doi: 10.1016/0005-2736(88)90211-8. [DOI] [PubMed] [Google Scholar]
  19. Simpson R. J., Peters T. J. Iron-binding lipids of rabbit duodenal brush-border membrane. Biochim Biophys Acta. 1987 Apr 9;898(2):181–186. doi: 10.1016/0005-2736(87)90036-8. [DOI] [PubMed] [Google Scholar]
  20. Simpson R. J., Peters T. J. Transport of Fe2+ across lipid bilayers: possible role of free fatty acids. Biochim Biophys Acta. 1987 Apr 9;898(2):187–195. doi: 10.1016/0005-2736(87)90037-x. [DOI] [PubMed] [Google Scholar]
  21. Stedman R. L. The chemical composition of tobacco and tobacco smoke. Chem Rev. 1968 Apr;68(2):153–207. doi: 10.1021/cr60252a002. [DOI] [PubMed] [Google Scholar]
  22. van der Vusse G. J., Reneman R. S. The myocardial non-esterified fatty acid controversy. J Mol Cell Cardiol. 1984 Aug;16(8):677–682. doi: 10.1016/s0022-2828(84)80652-5. [DOI] [PubMed] [Google Scholar]

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